α-Synuclein E46K Mutation and Involvement of Oxidative Stress in a Drosophila Model of Parkinson’s Disease

Figure 7

SNCA^E46K transgenic flies move less and slower. An automated fly tracking using iFLY apparatus demonstrates the lower general movement of SNCA^E46K/+; Ddc/+ when compared to the control genotype SNCA^E46K/+. The position of the control flies in the first 2,699 frames (a) is compared to the position of SNCA^WT expressing flies (b). The heat map figure shows a decline in locomotor activity among the PD model transgenic flies. Position histogram of 2,699 frames captured from control genotype, SNCA^E46K/+ (c), and that of SNCA^E46K/+; Ddc/+ genotype (d) indicates control flies spent more of their time on the top of the tube but are able to move across the whole tube. While SNCA transgenic Drosophila flies prefer to stay at the tube bottom and do not climb up. Lighter heat map spots indicate stationary objects.

(a)

(b)

(c)

(d)