α-Synuclein E46K Mutation and Involvement of Oxidative Stress in a Drosophila Model of Parkinson’s Disease

Table 1

Overexpression of human SNCA leads to oxidative stress.

Genotype	CAT	SOD	GSH	LPO	ROS
UAS-SNCA^WT/+	6.7 ± 0.32	0.58 ± 0.01	19.2 ± 2.03	6.1 ± 0.24	689 ± 25.6
elav/+; SNCA^WT/+	4.5 ± 0.24	0.49 ± 0.02	11.5 ± 1.50	7.0 ± 0.85	844 ± 11.3
value
UAS-SNCA^E46K/+	6.5 ± 0.27	0.62 ± 0.04	18.5 ± 1.34	5.0 ± 0.47	700 ± 33.1
elav/+; SNCA^E46K/+	4.4 ± 0.53	0.47 ± 0.03	12.8 ± 1.02	6.8 ± 0.26	895 ± 21.6
value

Endogenous antioxidant enzyme activity and levels of oxidative markers are noticeably distorted and in SNCA transgenic PD model flies. Data represent mean ± SE of each biochemical marker. An independent sample t-test was applied, and corresponding values indicate significant differences between tested genotypes. A total of 9 samples were analyzed in each genotype (n = 9). CAT = catalase-specific activity (μM H₂O₂/min/mg protein); SOD = superoxide-dismutase-specific activity (units/mg protein); GSH = glutathione levels (μg GSH/mg protein); LPO = lipid peroxidation levels (nM MDA/mg protein); ROS = reactive oxygen species levels (μM DCF/mg protein).