Figure 3: ChIP analysis of ST2 cells treated with/without BMP2 and/or TRO. (a) After ST2 cells were cultured under the conditions described in Figure 2, cells were lysed and ChIP analysis was performed according to the manufacturer’s protocol (Millipore) using the indicated antibodies. H3KAc, anti-acetylated Lys histone H3 (Millipore); H3K4me3, anti-trimethylated Lys4 histone H3 (Millipore). Samples were examined by qPCR using CFX96 (Bio-Rad). Primers were used as previously reported [19]. Each experiment was performed at least three times. Student’s t-tests were performed. . (b) ChIP analysis of ST2 cells using anti-H3AcK, -H3K4me3, and -PPARγ. ChIP assays were performed under the same conditions as (a). PCR was performed as described and visualized on 2% agarose TAE gels.