Signaling Crosstalk between PPARγ and BMP2 in Mesenchymal Stem Cells
Figure 2
Adipocyte and osteoblast marker gene mRNA levels were altered by changing the order of BMP2 and TRO treatment. (a) Scheme of experiments used in Figures 2 and 3. We examined five conditions. (-) Untreated ST2 cells were cultured in αMEM supplemented with 10% FBS and penicillin/streptomycin. (TRO) ST2 cells were incubated with one TRO for three hr. (BMP) ST2 cells were treated with 50 ng/mL BMP2 for three hr. (TRO-BMP) ST2 cells were treated with TRO for three hr, the medium was changed, and the cells were then incubated with BMP2 for three hr. (BMP-TRO) ST2 cells were treated with BMP2 for three hr and the medium was changed; the cells were then incubated with TRO for three hr. (b) RT-qPCR analyses of adipocyte or osteoblast differentiation marker genes. After ST2 cells were cultured under the conditions described above, cells were collected and RNAs were extracted. Then RT-qPCR experiments were performed and normalized to Gapdh mRNA. Each experiment was performed at least three times. Student’s t-test was performed. .
