Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose
Effect of TOFyl-COA on fluorescence emission characteristics of L-FABP-bound NBD-stearic acid. NBD-stearic acid, bound to L-FABP as in Figure 3, was excited at 490 nm and fluorescence emission spectra obtained before and after addition of TOFyl-CoA (Methods). Panel (a): fluorescence emission spectra of L-FABP- (25 nM) bound NBD stearate (80 nM) without (filled circles) and with (open circles) of TOFyl-CoA (1500 nM). With increasing TOFyl-CoA concentration, the emission maximum of L-FABP-bound NBD stearate shifted to lower wavelength (panel b), and fluorescence intensity increased (panel c).