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Volume 2012, Article ID 609572, 4 pages
Research Article

Age-related and Individual Variation in Male Piezodorus hybneri (Heteroptera: Pentatomidae) Pheromones

1Agro-Environment Research Division, NARO Kyushu Okinawa Agricultural Research Center (KARC), 2421 Suya, Koshi, Kumamoto 861-1192, Japan
2Division of Plant Protection, NARO Agricultural Research Center (NARC), 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan
3Ecomone Division, Fuji Flavor Co., Ltd., 3-5-8 Midorigaoka, Hamura, Tokyo 205-8503, Japan

Received 15 September 2011; Revised 7 December 2011; Accepted 29 December 2011

Academic Editor: Jeffrey R. Aldrich

Copyright © 2012 Nobuyuki Endo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Males of the Piezodorus hybneri stink bug produce a pheromone comprising β-sesquiphellandrene (Sesq), (R)-15-hexadecanolide (R15), and methyl (Z)-8-hexadecenoate (Z8). We collected airborne volatiles from individual P. hybneri males and analyzed them by GC-MS. Daily analysis from 1 to 16 days after adult emergence showed that pheromone emission started around 3 to 6 days after adult emergence and peaked (~1 μg/male/day) on day 11. The proportion of Sesq tended to increase with age to about 80% on days 12 to 16. On the other hand, the proportion of R15 tended to decrease with age. The proportion of Z8 reached a maximum of about 34% on day 9 but otherwise remained below 20%. The total amount of pheromone emitted by individual males varied considerably: three males emitted more than 10 μg, whereas another three males emitted little or no pheromone and failed to survive by the end of the experiment. These results suggest that the amount of P. hybneri pheromone and its blend ratio could be affected by the male’s physical conditions, such as vitality and age.