Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2011 (2011), Article ID 434392, 8 pages
http://dx.doi.org/10.4061/2011/434392
Review Article

Optimization Manufacture of Virus- and Tumor-Specific T Cells

Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX77030, USA

Received 26 April 2011; Accepted 20 June 2011

Academic Editor: Anna Rita Migliaccio

Copyright © 2011 Natalia Lapteva and Juan F. Vera. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Although ex vivo expanded T cells are currently widely used in pre-clinical and clinical trials, the complexity of manufacture remains a major impediment for broader application. In this review we discuss current protocols for the ex vivo expansion of virus- and tumor-specific T cells and describe our experience in manufacture optimization using a gas-permeable static culture flask (G-Rex). This innovative device has revolutionized the manufacture process by allowing us to increase cell yields while decreasing the frequency of cell manipulation and in vitro culture time. It is now being used in good manufacturing practice (GMP) facilities for clinical cell production in our institution as well as many others in the US and worldwide.