Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NOD/SCID/IL2R𝜸^null mice

Figure 4

At day 4 after transfusion, human EBs are mostly detectable in spleen of NOD/SCID/IL2R𝛾^null mice. (a) Flow cytometric analysis for human CD235a and CFSE expression of bone marrow and spleen cells from transfused and nontransfused (negative control) NOD/SCID/IL2R𝛾^null mice. The results are representative of those obtained with 2 mice per group (see Table 2 for further details) and are representative of those observed in three separate experiments. (b) Immunocytochemistry for human CD235a of cells from the spleen of untransfused (top left panel) and transfused (lower panels) mice sacrificed 4 days after the human EBs transfusion, as indicated. Cells from control mice (top left panel) are negative while cultured human EBs (top right panel) are all positive. In transfused mice, human CD235a^pos cells (arrows) represented up to 18.2±5.6% of total splenic cells. However, they were trapped inside larger CD235a^neg cells, probably macrophages of murine origin. Magnifications 40x and 100x, as indicated.