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Stem Cells International
Volume 2011, Article ID 765378, 9 pages
http://dx.doi.org/10.4061/2011/765378
Research Article

Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos

1Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, NY 10032, USA
2Department of Biomedical Engineering, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
3Department of Obstetrics and Gynecology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
4Division of Experimental Therapeutics, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA

Received 14 December 2010; Accepted 17 March 2011

Academic Editor: Qiang Feng

Copyright © 2011 Svetlana Gavrilov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480 embryos not suitable for clinical use to assess grading criteria indicative of loss of viability on ED5. Our analysis indicated that a large proportion of in vitro fertilization (IVF) embryos not suitable for clinical use could be used for hESC derivation. Based on these combined findings, we propose that criteria commonly used in IVF clinics to determine optimal embryos for uterine transfer can be employed to predict the potential for hESC derivation from poor quality embryos without the destruction of vital human embryos.