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Stem Cells International
Volume 2011 (2011), Article ID 765378, 9 pages
http://dx.doi.org/10.4061/2011/765378
Research Article

Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos

1Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, NY 10032, USA
2Department of Biomedical Engineering, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
3Department of Obstetrics and Gynecology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
4Division of Experimental Therapeutics, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA

Received 14 December 2010; Accepted 17 March 2011

Academic Editor: Qiang Feng

Copyright © 2011 Svetlana Gavrilov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1. Karyotypes of CU1 (passage 9) (A) and CU2 (passage 8) (B). Verbatim lab reports.

Figure S2. Characterization of CHB1 (A-J) and CU2 (K-O) hESC lines. Immunohistochemical analysis with DAPI counterstain revealed that hESC colonies expressed pluripotency markers POU5F1/OCT4 (A,K), SSEA-4 (B,L), TRA1-60 (D), TRA-1-81 (E) (green fluorescence) and were negative for differentiation marker SSEA-1 (C). hESC cultured as embryoid bodies differentiated into all three germ layers: hematoxylin and eosin (F) and Masson's trichrome (G), immunohistochemistry for neuron specific beta-III tubulin (TUJ-1) (H,M), alpha fetoprotein (AFP) (I,N) and smooth muscle actin (SMA) (J,O) (green fluorescence). Scale bar-100 μm.

  1. Supplementary Figure 1
  2. Supplementary Figure 2