Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media

Figure 2

Cultivation of nonadherent cell fractions removed from the culture of primary BM MNCs in different media. Nonadherent cells and spent medium removed from each of the flasks, demonstrated in Figure 1, were replated into a new T-25 flask coated with gelatin containing 4 mL of the fresh medium—that is, Mesencult-XF (a), StemPro MSC SFM Xeno-Free (b), MSCGM-CD (c), PPRF-msc6 (d), and 10% FBS DMEM (e). After 60 h, nonadherent cells and medium in each flask were discarded, and fresh medium was added to the adherent cells (8 mL per flask). The adherent cells were allowed to grow for additional 8 days with 50% medium change every other day and then stained with crystal violet to visualize colonies generated.