Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2012, Article ID 414038, 13 pages
http://dx.doi.org/10.1155/2012/414038
Research Article

Directed Fusion of Mesenchymal Stem Cells with Cardiomyocytes via VSV-G Facilitates Stem Cell Programming

1Department of Biomedical Engineering, University of Wisconsin at Madison, Madison, WI 53706, USA
2Department of Pathobiological Sciences, University of Wisconsin at Madison, Madison, WI 53711, USA
3Department of Medicine, University of Wisconsin at Madison, Madison, WI 53706, USA
4The Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, WI 53706, USA
5The Material Sciences Program, University of Wisconsin at Madison, Madison, WI 53706, USA

Received 19 January 2012; Accepted 22 February 2012

Academic Editor: Selim Kuçi

Copyright © 2012 Nicholas A. Kouris et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary information contains representative images of MSC-CM fusion products and corresponding fusion partners (MSCs and CMs) following immunocytochemistry for expression of CD73, CD90, CD105 and sarcomeric myosin proteins. Images show that MSC-CM fusion products can contain two or more nuclei or a single (sometimes enlarged) nucleus.

Supplementary Figure 1. Characterization of phenotype of human mesenchymal stem cells and HL-1 cardiomyocytes and vMSC-CM fusion products. A) MSC and CM populations used for these studies expressed CD73 (green) and CD90 (green), while only MSCs expressed CD105 (green) and only CMs expressed MF20 (red). Shown are representative images of cells following immunofluorescence labeling for each respective marker (+) or secondary antibody only (-) and counterstained with DAPI (blue). Scale bar = 50 μm. B) Representative CD105+/MF20+ cells (white arrows) following pH-induced fusion of vMSC and CM. Scale bar = 50 μm.

  1. Supplementary Figure