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Stem Cells International
Volume 2012, Article ID 652034, 4 pages
http://dx.doi.org/10.1155/2012/652034
Review Article

Safety of Mesenchymal Stem Cells for Clinical Application

1State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union of Medical College, Tianjin 300020, China
2Department of Hematology, Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou 450052, China

Received 18 February 2012; Accepted 13 March 2012

Academic Editor: Selim Kuçi

Copyright © 2012 Youwei Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Hayes, G. Curley, and J. G. Laffey, “Mesenchymal stem cells—a promising therapy for Acute Respiratory Distress Syndrome,” F1000 Medicine Reports, vol. 4, p. 2, 2012. View at Google Scholar
  2. S. Ishikane, S. Ohnishi, K. Yamahara et al., “Allogeneic injection of fetal membrane-derived mesenchymal stem cells induces therapeutic angiogenesis in a rat model of hind limb ischemia,” Stem Cells, vol. 26, no. 10, pp. 2625–2633, 2008. View at Publisher · View at Google Scholar · View at Scopus
  3. H. Nakajima, K. Uchida, A. Rodriguez et al., “Transplantation of mesenchymal stem cells promotes the alternative pathway of macrophage activation and functional recovery after spinal cord injury,” Journal of Neurotrauma. In press.
  4. M. A. Puglisi, V. Tesori, W. Lattanzi et al., “Therapeutic implications of mesenchymal stem cells in liver injury,” Journal of Biomedicine and Biotechnology, vol. 2011, Article ID 860578, 2011. View at Google Scholar
  5. L. L. Lu, Y. J. Liu, S. G. Yang et al., “Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials,” Haematologica, vol. 91, no. 8, pp. 1017–1026, 2006. View at Google Scholar · View at Scopus
  6. A. I. Caplan, “Why are MSCs therapeutic? New data: new insight,” Journal of Pathology, vol. 217, no. 2, pp. 318–324, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. A. Maitra, D. E. Arking, N. Shivapurkar et al., “Genomic alterations in cultured human embryonic stem cells,” Nature Genetics, vol. 37, no. 10, pp. 1099–1103, 2005. View at Publisher · View at Google Scholar · View at Scopus
  8. E. Närvä, R. Autio, N. Rahkonen et al., “High-resolution DNA analysis of human embryonic stem cell lines reveals culture-induced copy number changes and loss of heterozygosity,” Nature Biotechnology, vol. 28, no. 4, pp. 371–377, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. J. J. Buzzard, N. M. Gough, J. M. Crook, and A. Colman, “Karyotype of human ES cells during extended culture,” Nature Biotechnology, vol. 22, no. 4, pp. 381–382, 2004. View at Publisher · View at Google Scholar
  10. J. S. Draper, K. Smith, P. Gokhale et al., “Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells,” Nature Biotechnology, vol. 22, no. 1, pp. 53–54, 2004. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Inzunza, S. Sahlén, K. Holmberg et al., “Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation,” Molecular Human Reproduction, vol. 10, no. 6, pp. 461–466, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. D. E. C. Baker, N. J. Harrison, E. Maltby et al., “Adaptation to culture of human embryonic stem cells and oncogenesis in vivo,” Nature Biotechnology, vol. 25, no. 2, pp. 207–215, 2007. View at Publisher · View at Google Scholar · View at Scopus
  13. L. C. Laurent, I. Ulitsky, I. Slavin et al., “Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture,” Cell Stem Cell, vol. 8, no. 1, pp. 106–118, 2011. View at Publisher · View at Google Scholar · View at Scopus
  14. M. E. Bernardo, N. Zaffaroni, F. Novara et al., “Human bone marrow-derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms,” Cancer Research, vol. 67, no. 19, pp. 9142–9149, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. A. Schellenberg, Q. Lin, H. Schuler et al., “Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks,” Aging, vol. 3, no. 9, pp. 873–888, 2011. View at Google Scholar
  16. A. Torsvik, G. V. Rosland, and R. Bjerkvig, “Spontaneous transformation of adult mesenchymal stem cells from cynomolgus macaques in vitro,” Experimental Cell Research, vol. 317, no. 20, pp. 2950–2957, 2011. View at Google Scholar
  17. Z. Ren et al., “Spontaneous transformation of mesenchymal stem cells in culture: facts or fiction?” Experimental Cell Research. In press.
  18. W. Wu, Q. He, X. Li et al., “Long-term cultured human neural stem cells undergo spontaneous transformation to tumor-initiating cells,” International Journal of Biological Sciences, vol. 7, no. 6, pp. 892–901, 2011. View at Google Scholar · View at Scopus
  19. G. V. Røsland, A. Svendsen, A. Torsvik et al., “Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation,” Cancer Research, vol. 69, no. 13, pp. 5331–5339, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Garcia, M. C. Martín, R. de la Fuente, J. C. Cigudosa, J. Garcia-Castro, and A. Bernad, “Pitfalls in spontaneous in vitro transformation of human mesenchymal stem cells,” Experimental Cell Research, vol. 316, no. 9, pp. 1648–1650, 2010. View at Publisher · View at Google Scholar · View at Scopus
  21. R. De La Fuente, A. Bernad, J. Garcia-Castro, M. C. Martin, and J. C. Cigudosa, “Retraction: spontaneous human adult stem cell transformation,” Cancer Research, vol. 70, no. 16, p. 6682, 2010. View at Publisher · View at Google Scholar · View at Scopus
  22. O. A. Buyanovskaya, N. P. Kuleshov, V. A. Nikitina, E. S. Voronina, L. D. Katosova, and N. P. Bochkov, “Spontaneous aneuploidy and clone formation in adipose tissue stem cells during different periods of culturing,” Bulletin of Experimental Biology and Medicine, vol. 148, no. 1, pp. 109–112, 2009. View at Publisher · View at Google Scholar · View at Scopus
  23. D. Rubio, J. Garcia-Castro, M. C. Martín et al., “Spontaneous human adult stem cell transformation,” Cancer Research, vol. 65, no. 8, pp. 3035–3039, 2005. View at Google Scholar · View at Scopus
  24. A. Torsvik, G. V. Røsland, A. Svendsen et al., “Spontaneous malignant transformation of human mesenchymal stem cells reflects cross-contamination: putting the research field on track—letter,” Cancer Research, vol. 70, no. 15, pp. 6393–6396, 2010. View at Publisher · View at Google Scholar · View at Scopus
  25. Z. Ren, J. Wang, w. Zhu et al., “Spontaneous transformation of adult mesenchymal stem cells from cynomolgus macaques in vitro,” Experimental Cell Research, vol. 317, no. 20, pp. 2950–2957, 2011. View at Google Scholar
  26. K. Tian, S. Yang, Q. Ren et al., “P38 MAPK contributes to the growth inhibition of leukemic tumor cells mediated by human umbilical cord mesenchymal stem cells,” Cellular Physiology and Biochemistry, vol. 26, no. 6, pp. 799–808, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. Y. Wang, Z. B. Han, J. Ma et al., “A toxicity study of multiple-administration human umbilical cord mesenchymal stem cells in cynomolgus monkeys,” Stem Cells and Development. In press.
  28. C. J. Centeno, J. R. Schultz, M. Cheever, B. Robinson, M. Freeman, and W. Marasco, “Safety and complications reporting on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique,” Current Stem Cell Research and Therapy, vol. 5, no. 1, pp. 81–93, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. K. Tarte, J. Gaillard, J. J. Lataillade et al., “Clinical-grade production of human mesenchymal stromal cells: occurrence of aneuploidy without transformation,” Blood, vol. 115, no. 8, pp. 1549–1553, 2010. View at Publisher · View at Google Scholar · View at Scopus
  30. K. Cooper and C. Viswanathan, “Establishment of a mesenchymal stem cell bank,” Stem Cells International, vol. 2011, Article ID 905621, 8 pages, 2011. View at Publisher · View at Google Scholar
  31. W. Gong, Z. Han, H. Zhao et al., “Banking human umbilical cord derived mesenchymal stromal cells for clinical use,” Cell Transplantation, vol. 21, no. 1, pp. 207–216, 2012. View at Google Scholar
  32. S. Thirumala, W. S. Goebel, and E. J. Woods, “Clinical grade adult stem cell banking,” Organogenesis, vol. 5, no. 3, pp. 143–154, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. G. Liu, H. Zhou, Y. Li et al., “Evaluation of the viability and osteogenic differentiation of cryopreserved human adipose-derived stem cells,” Cryobiology, vol. 57, no. 1, pp. 18–24, 2008. View at Publisher · View at Google Scholar · View at Scopus
  34. J. Reinhardt, A. Stuhler, and J. Blumel, “Safety of bovine sera for production of mesenchymal stem cells for therapeutic use,” Human Gene Therapy, vol. 22, no. 6, p. 775, 2011. View at Google Scholar
  35. R. Crespo-Diaz, A. Behfar, G. W. Butler et al., “Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability,” Cell Transplantation, vol. 20, no. 6, pp. 797–811, 2011. View at Google Scholar
  36. R. A. Woo and R. Y. C. Poon, “Activated oncogenes promote and cooperate with chromosomal instability for neoplastic transformation,” Genes and Development, vol. 18, no. 11, pp. 1317–1330, 2004. View at Publisher · View at Google Scholar · View at Scopus