Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2012, Article ID 841203, 11 pages
http://dx.doi.org/10.1155/2012/841203
Research Article

Development of a Vascularized Skin Construct Using Adipose-Derived Stem Cells from Debrided Burned Skin

1Dental and Trauma Research Detachment, United States Army Institute of Surgical Research, Fort Sam Houston, TX 78234-6315, USA
2Department of Extremity Trauma Research and Regenerative Medicine, United States Army Institute of Surgical Research, Fort Sam Houston, TX 78234-6315, USA
3Burn Center, United States Army Institute of Surgical Research, Fort Sam Houston, TX 78234-6315, USA

Received 21 February 2012; Accepted 25 May 2012

Academic Editor: Roland Meisel

Copyright © 2012 Rodney K. Chan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. B. D. Owens, J. F. Kragh Jr., J. C. Wenke, J. Macaitis, C. E. Wade, and J. B. Holcomb, “Combat wounds in operation Iraqi Freedom and operation Enduring Freedom,” The Journal of Trauma and Acute Care Surgery, vol. 64, no. 2, pp. 295–299, 2008. View at Google Scholar · View at Scopus
  2. S. E. Wolf, D. S. Kauvar, C. E. Wade et al., “Comparison between civilian burns and combat burns from Operation Iraqi Freedom and Operation Enduring Freedom,” Annals of Surgery, vol. 243, no. 6, pp. 786–795, 2006. View at Publisher · View at Google Scholar · View at Scopus
  3. “Nationial burn repository 2010 report,” American Burn Association, National Burn Repository, Version 6.0, 2010.
  4. D. Mellus and R. K. Chan, “Reconstructive surgery in the thermally injured patient,” Perioperative Nursing Clinics, vol. 7, no. 1, pp. 107–113, 2012. View at Google Scholar
  5. M. G. Jeschke, R. P. Mlcak, C. C. Finnerty et al., “Burn size determines the inflammatory and hypermetabolic response,” Critical Care, vol. 11, no. 4, article R90, 2007. View at Publisher · View at Google Scholar · View at Scopus
  6. R. Kraft, D. N. Herndon, A. M. Al-Mousawi, F. N. Williams, C. C. Finnerty, and M. G. Jeschke, “Burn size and survival probability in paediatric patients in modern burn care: a prospective observational cohort study,” The Lancet, vol. 379, no. 9820, pp. 1013–1021, 2012. View at Google Scholar
  7. J. K. Cole, L. H. Engrav, D. M. Heimbach et al., “Early excision and grafting of face and neck burns in patients over 20 years,” Plastic and Reconstructive Surgery, vol. 109, no. 4, pp. 1266–1273, 2002. View at Google Scholar · View at Scopus
  8. M. B. Donelan, “Principles of burn reconstruction, in plastic surgery,” in Grabb and Smith's Plastic Surgery, C. H. Thorne, R. W. Beasley, S. J. Aston et al., Eds., Lippincott Williams & Wilkins, Philadelphia, Pa, USA, 6 edition, 2007. View at Google Scholar
  9. M. B. Klein, “Thermal, chemical and electrical injuries, in plastic surgery,” in Grabb and Smith's Plastic Surgery, C. H. Thorne, R. W. Beasley, S. J. Aston et al., Eds., pp. 132–149, Lippincott Williams & Wilkins, Philadelphia, Pa, USA, 6 edition, 2007. View at Google Scholar
  10. D. L. Larson, “Closure of the burn wound,” The Journal of Trauma and Acute Care Surgery, vol. 5, no. 2, pp. 254–266, 1965. View at Google Scholar · View at Scopus
  11. D. L. Larson and S. Abston, “Acutely burned patient. Initial care and closure of burn wound,” New York State Journal of Medicine, vol. 70, no. 12, pp. 1626–1633, 1970. View at Google Scholar · View at Scopus
  12. J. B. Lundy, L. C. Cancio, B. T. King, S. E. Wolf, E. M. Renz, and L. H. Blackbourne, “Experience with the use of close-relative allograft for the management of extensive thermal injury in local national casualties during Operation Iraqi Freedom,” American Journal of Disaster Medicine, vol. 6, no. 5, pp. 319–324, 2011. View at Google Scholar
  13. V. C. van der Veen, B. K. H. L. Boekema, M. M. W. Ulrich, and E. Middelkoop, “New dermal substitutes,” Wound Repair and Regeneration, vol. 19, supplement 1, pp. S59–S65, 2011. View at Publisher · View at Google Scholar · View at Scopus
  14. I. V. Yannas, E. Lee, D. P. Orgill, E. M. Skrabut, and G. F. Murphy, “Synthesis and characterization of a model extracellular matrix that induces partial regeneration of adult mammalian skin,” Proceedings of the National Academy of Sciences of the United States of America, vol. 86, no. 3, pp. 933–937, 1989. View at Google Scholar · View at Scopus
  15. I. V. Yannas, D. P. Orgill, and J. F. Burke, “Template for skin regeneration,” Plastic and Reconstructive Surgery, vol. 127, supplement 1, pp. 60S–70S, 2011. View at Publisher · View at Google Scholar
  16. R. Sood, D. Roggy, M. Zieger et al., “Cultured epithelial autografts for coverage of large burn wounds in eighty-eight patients: the Indiana university experience,” Journal of Burn Care & Research, vol. 31, no. 4, pp. 559–568, 2010. View at Publisher · View at Google Scholar · View at Scopus
  17. M. B. Klein, L. H. Engrav, J. H. Holmes et al., “Management of facial burns with a collagen/glycosaminoglycan skin substitute—prospective experience with 12 consecutive patients with large, deep facial burns,” Burns, vol. 31, no. 3, pp. 257–261, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. C. E. Butler, I. V. Yannas, C. C. Compton, C. A. Correia, and D. P. Orgill, “Comparison of cultured and uncultured keratinocytes seeded into a collagen-GAG matrix for skin replacements,” British Journal of Plastic Surgery, vol. 52, no. 2, pp. 127–132, 1999. View at Publisher · View at Google Scholar · View at Scopus
  19. C. E. Butler, D. P. Orgill, I. V. Yannas, and C. C. Compton, “Effect of keratinocyte seeding of collagen-glycosaminoglycan membranes on the regeneration of skin in a porcine model,” Plastic and Reconstructive Surgery, vol. 101, no. 6, pp. 1572–1579, 1998. View at Publisher · View at Google Scholar · View at Scopus
  20. D. P. Orgill, C. Butler, J. F. Regan, M. S. Barlow, I. V. Yannas, and C. C. Compton, “Vascularized collagen-glycosaminoglycan matrix provides a dermal substrate and improves take of cultured epithelial autografts,” Plastic and Reconstructive Surgery, vol. 102, no. 2, pp. 423–429, 1998. View at Publisher · View at Google Scholar · View at Scopus
  21. S. Natesan, N. L. Wrice, D. G. Baer, and R. J. Christy, “Debrided skin as a source of autologous stem cells for wound repair,” Stem Cells, vol. 29, no. 8, pp. 1219–1230, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. V. Trottier, G. Marceau-Fortier, L. Germain, C. Vincent, and J. Fradette, “IFATS collection: using human adipose-derived stem/stromal cells for the production of new skin substitutes,” Stem Cells, vol. 26, no. 10, pp. 2713–2723, 2008. View at Publisher · View at Google Scholar · View at Scopus
  23. L. K. Barthel and P. A. Raymond, “Improved method for obtaining 3-microns cryosections for immunocytochemistry,” Journal of Histochemistry & Cytochemistry, vol. 38, no. 9, pp. 1383–1388, 1990. View at Google Scholar · View at Scopus
  24. S. Natesan, G. Zhang, D. G. Baer, T. J. Walters, R. J. Christy, and L. J. Suggs, “A bilayer construct controls adipose-derived stem cell differentiation into endothelial cells and pericytes without growth factor stimulation,” Tissue Engineering A, vol. 17, no. 7-8, pp. 941–953, 2011. View at Publisher · View at Google Scholar · View at Scopus
  25. P. A. Zuk, M. Zhu, P. Ashjian et al., “Human adipose tissue is a source of multipotent stem cells,” Molecular Biology of the Cell, vol. 13, no. 12, pp. 4279–4295, 2002. View at Publisher · View at Google Scholar · View at Scopus
  26. J. L. Fletcher, E. J. Caterson, R. G. Hale, L. C. Cancio, E. M. Renz, and R. K. Chan, “Characterization of skin allograft use in thermal injury,” Journal of Burn Care & Research. In press.
  27. M. Crisan, S. Yap, L. Casteilla et al., “A perivascular origin for mesenchymal stem cells in multiple human organs,” Cell Stem Cell, vol. 3, no. 3, pp. 301–313, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. D. O. Traktuev, S. Merfeld-Clauss, J. Li et al., “A population of multipotent CD34-positive adipose stromal cells share pericyte and mesenchymal surface markers, reside in a periendothelial location, and stabilize endothelial networks,” Circulation Research, vol. 102, no. 1, pp. 77–85, 2008. View at Publisher · View at Google Scholar · View at Scopus
  29. B. A. Bunnell, B. T. Estes, F. Guilak, and J. M. Gimble, “Differentiation of adipose stem cells,” Methods in Molecular Biology, vol. 456, pp. 155–171, 2008. View at Publisher · View at Google Scholar · View at Scopus
  30. A. J. Katz, A. Tholpady, S. S. Tholpady, H. Shang, and R. C. Ogle, “Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells,” Stem Cells, vol. 23, no. 3, pp. 412–423, 2005. View at Publisher · View at Google Scholar · View at Scopus
  31. P. A. Zuk, M. Zhu, H. Mizuno et al., “Multilineage cells from human adipose tissue: implications for cell-based therapies,” Tissue Engineering, vol. 7, no. 2, pp. 211–228, 2001. View at Publisher · View at Google Scholar · View at Scopus
  32. L. Aust, B. Devlin, S. J. Foster et al., “Yield of human adipose-derived adult stem cells from liposuction aspirates,” Cytotherapy, vol. 6, no. 1, pp. 7–14, 2004. View at Publisher · View at Google Scholar · View at Scopus
  33. B. Prunet-Marcassus, B. Cousin, D. Caton, M. André, L. Pénicaud, and L. Casteilla, “From heterogeneity to plasticity in adipose tissues: site-specific differences,” Experimental Cell Research, vol. 312, no. 6, pp. 727–736, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. A. Cirodde, T. Leclerc, P. Jault, P. Duhamel, J. J. Lataillade, and L. Bargues, “Cultured epithelial autografts in massive burns: a single-center retrospective study with 63 patients,” Burns, vol. 37, no. 6, pp. 964–972, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. H. Yim, H. T. Yang, Y. S. Cho et al., “Clinical study of cultured epithelial autografts in liquid suspension in severe burn patients,” Burns, vol. 37, no. 6, pp. 1067–1071, 2011. View at Publisher · View at Google Scholar · View at Scopus
  36. B. S. Atiyeh and M. Costagliola, “Cultured epithelial autograft (CEA) in burn treatment: three decades later,” Burns, vol. 33, no. 4, pp. 405–413, 2007. View at Publisher · View at Google Scholar · View at Scopus
  37. G. Gravante, M. C. Di Fede, A. Araco et al., “A randomized trial comparing ReCell system of epidermal cells delivery versus classic skin grafts for the treatment of deep partial thickness burns,” Burns, vol. 33, no. 8, pp. 966–972, 2007. View at Publisher · View at Google Scholar · View at Scopus
  38. F. M. Wood, M. L. Stoner, B. V. Fowler, and M. W. Fear, “The use of a non-cultured autologous cell suspension and Integra dermal regeneration template to repair full-thickness skin wounds in a porcine model: a one-step process,” Burns, vol. 33, no. 6, pp. 693–700, 2007. View at Publisher · View at Google Scholar · View at Scopus
  39. S. T. Boyce, “Design principles for composition and performance of cultured skin substitutes,” Burns, vol. 27, no. 5, pp. 523–533, 2001. View at Publisher · View at Google Scholar · View at Scopus
  40. A. D. Metcalfe and M. W. J. Ferguson, “Bioengineering skin using mechanisms of regeneration and repair,” Biomaterials, vol. 28, no. 34, pp. 5100–5113, 2007. View at Publisher · View at Google Scholar · View at Scopus
  41. A. D. Metcalfe and M. W. J. Ferguson, “Tissue engineering of replacement skin: the crossroads of biomaterials, wound healing, embryonic development, stem cells and regeneration,” Journal of the Royal Society Interface, vol. 4, no. 14, pp. 413–417, 2007. View at Publisher · View at Google Scholar · View at Scopus
  42. C. Auxenfans, C. Lequeux, E. Perrusel, A. Mojallal, B. Kinikoglu, and O. Damour, “Adipose-derived stem cells (ASCs) as a source of endothelial cells in the reconstruction of endothelialized skin equivalents,” Journal of Tissue Engineering and Regenerative Medicine. In press. View at Publisher · View at Google Scholar · View at Scopus
  43. P. A. Janmey, J. P. Winer, and J. W. Weisel, “Fibrin gels and their clinical and bioengineering applications,” Journal of the Royal Society Interface, vol. 6, no. 30, pp. 1–10, 2009. View at Publisher · View at Google Scholar · View at Scopus
  44. F. M. Shaikh, A. Callanan, E. G. Kavanagh, P. E. Burke, P. A. Grace, and T. M. McGloughlin, “Fibrin: a natural biodegradable scaffold in vascular tissue engineering,” Cells Tissues Organs, vol. 188, no. 4, pp. 333–346, 2008. View at Publisher · View at Google Scholar · View at Scopus
  45. O. Skalli, M. F. Pelte, M. C. Peclet et al., “Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes,” Journal of Histochemistry & Cytochemistry, vol. 37, no. 3, pp. 315–321, 1989. View at Google Scholar · View at Scopus
  46. S. Merfeld-Clauss, N. Gollahalli, K. L. March, and D. O. Traktuev, “Adipose tissue progenitor cells directly interact with endothelial cells to induce vascular network formation,” Tissue Engineering A, vol. 16, no. 9, pp. 2953–2966, 2010. View at Publisher · View at Google Scholar · View at Scopus
  47. S. M. Lam, “Fat transfer for the management of soft tissue trauma: the do's and the don'ts,” Facial Plastic Surgery, vol. 26, no. 6, pp. 488–493, 2010. View at Publisher · View at Google Scholar · View at Scopus
  48. M. Wetterau, C. Szpalski, A. Hazen, and S. M. Warren, “Autologous fat grafting and facial reconstruction,” The Journal of Craniofacial Surgery, vol. 23, no. 1, pp. 315–318, 2012. View at Publisher · View at Google Scholar
  49. C. Hrabchak, L. Flynn, and K. A. Woodhouse, “Biological skin substitutes for wound cover and closure,” Expert Review of Medical Devices, vol. 3, no. 3, pp. 373–385, 2006. View at Publisher · View at Google Scholar · View at Scopus
  50. Z. Ruszczak, “Effect of collagen matrices on dermal wound healing,” Advanced Drug Delivery Reviews, vol. 55, no. 12, pp. 1595–1611, 2003. View at Publisher · View at Google Scholar · View at Scopus
  51. A. M. Altman, N. Matthias, Y. Yan et al., “Dermal matrix as a carrier for in vivo delivery of human adipose-derived stem cells,” Biomaterials, vol. 29, no. 10, pp. 1431–1442, 2008. View at Publisher · View at Google Scholar · View at Scopus
  52. S. Seetharaman, S. Natesan, R. S. Stowers et al., “A PEGylated fibrin-based wound dressing with antimicrobial and angiogenic activity,” Acta Biomaterialia, vol. 7, no. 7, pp. 2787–2796, 2011. View at Publisher · View at Google Scholar · View at Scopus