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Stem Cells International
Volume 2013, Article ID 582527, 9 pages
Clinical Study

Intracoronary Infusion of Autologous CD133+ Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell Homing

1Avicenna Tajik State Medical University, Dushanbe, Tajikistan
2Institute of Gastroenterology, 734003 Dushanbe, Tajikistan
3Tajikistan Ministry of Health, 734003 Dushanbe, Tajikistan
4UMRS 872, CRC-INSERM, Université Pierre et Marie Curie, Paris-VI, Université Paris Descartes, Paris-V, 15 rue de l’Ecole de Médecine, 75006 Paris, France

Received 11 January 2013; Revised 11 March 2013; Accepted 14 March 2013

Academic Editor: Weian Zhao

Copyright © 2013 Ubaidullo Kurbonov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


CD133 mesenchymal cells were enriched using magnetic microbead anti-CD133 antibody from bone marrow mononuclear cells (BMMNCs). Flow cytometry and immunocytochemistry analysis using specific antibodies revealed that these cells were essentially 89 ± 4% CD133+ and 8 ± 5% CD34+. CD133+/CD34+ BMMNCs secrete important bioactive proteins such as cardiotrophin-1, angiogenic and neurogenic factors, morphogenetic proteins, and proinflammatory and remodeling factors in vitro. Single intracoronary infusions of autologous CD133+/CD34+ BMMNCs are effective and reduce infarct size in patients as analyzed by Tc99m MIBI myocardial scintigraphy. The majority of patients were treated via left coronary artery. Nine months after cell therapy, 5 out of 8 patients showed a net positive response to therapy in different regions of the heart. Uptake of Tc99 isotope and revitalization of the heart area in inferoseptal region are more pronounced ( ) as compared to apex and anterosptal regions after intracoronary injection of the stem cells. The cells chosen here have the properties essential for their potential use in cell therapy and their homing can be followed without major difficulty by the scintigraphy. The cell therapy proposed here is safe and should be practiced, as we found, in conjunction with scintigraphic observation of areas of heart which respond optimally to the infusion of autologous CD133+/CD34+ BMMNCs.