Modifications of Human Subcutaneous ADMSC after PPARγ Activation and Cold Exposition

Figure 3

Effect of cold in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as described in Figure 1; total protein was isolated for detecting expression of adiponectin and FABP4 by western blot. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with . Differences between total and partial agonist in relation to control. # indicates meaningful differences observed after 4 hours of treatment with agonists after exposure to cold in comparison with adipocytes treated in equal conditions at 37°C. Data were normalized with GAPDH.

(a)

(b)