Analysis of the Pro- and Anti-Inflammatory Cytokines Secreted by Adult Stem Cells during Differentiation

Figure 1

Characterization of ASCs. (a) ASCs were cultured in ODM or ADM for 21 days and stained with alizarin red for osteogenesis or oil red o for adipogenesis. Representative images are shown. Original magnification for osteogenesis is 4x and that of adipogenesis is 10x. Scale bar represents 100 μm. Scale bar for insets represents 25 μm. (b) Cells were seeded at low density and incubated in CCM. After 14 days, colony-forming units were stained with crystal violet. A representative image is shown. (c) ASCs were stained with antibodies against the indicated antigens and analyzed by flow cytometry. Each colored line represents a specific donor ( donors), and respective isotype controls are shown as black lines.

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