Analysis of the Pro- and Anti-Inflammatory Cytokines Secreted by Adult Stem Cells during Differentiation

Table 1

qRT-PCR primer sequences for proinflammatory and anti-inflammatory cytokines.

Gene	Forward (5′→3′)	Reverse (5′→3′)
Proinflammatory cytokines
IL-1	CGCCAATGACTCAGAGGAAG	AGGGCGTCATTCAGGATCAA
IL-6	GTAGCCGCCCCACACAGACAGCC	GCCATCTTTGGAAGGTTC
IL-8	GAACTGAGAGTGATTGAGAGT	CTTCTCCACAACCCTCTG
IL-12	TGGAGTGCCAGGAGGACAGT	TCTTGGGTGGGTCAGGTTTG
IL-17	TGAAGGCAGGAATCACAAT	GGTGGATCGGTTGTAGTAAT
CCL8	CACAAGAATCACCAACATCC	TGGTCCAGATGCTTCATG
CXCL10	TCTGACTCTAAGTGGCATTC	ATTGTAGCAATGATCTCAACAC
G-CSF	AGCTTCCTGCTCAAGTGC	TTCTTCCATCTGCTGCCAGATGGT
ICAM-1	CACAGTCACCTATGGCAA	CTGGCTTCGTCAGAATCA
IFN-γ	TCAGCTCTGCATCGTTTTGG	GTTCCATTATCCGCTACATCTGAA
LIF	CCTGGACAAGCTATGTGG	GGTTGAGGATCTTCTGGTC
TNF-α	TCTTCTCGAACCCCGAGTGA	CCTCTGATGGCACCACCAG
Anti-inflammatory cytokines
IL-1RA	GTTCCATTCAGAGACGATCT	GTTGTTCCTCAGATAGAAGGT
IL-10	GTGATGCCCCAAGCTGAGA	CACGGCCTTGCTCTTGTTTT
IL-11	GGACCACAACCTGGATTC	GCAGGTAGGACAGTAGGT
IL-13	ATTGCTCTCACTTGCCTT	GTCAGGTTGATGCTCCAT
IL-18BP	ACCATGAGACACAACTGG	ATGCTGGACACTGCTTAG
IL-35	CACGTCCTTCATCCTCAG	GACTCCAGTCACTCAGTTC
CCL2	AGTCACCTGCTGTTATAACTT	CACAATGGTCTTGAAGATCAC
COX2	ACAGTCCACCAACTTACAAT	CAATCATCAGGCACAGGA
HGF	TTATCCTGACGTAAACACCTTTGATATAAC	CTGGGCAGTATTCGGGTTTGA
PTGES2	CCTGGAAGAGATCATCACC	CCTTCTCGTTGAGCATGA
STC-1	AGGATGATTGCTGAGGTG	TGTTATAGTATCTGTTGGAGAAGT
TGF-β	CAGCAACAATTCCTGGCGATA	AAGGCGAAAGCCCTCAATTT
TNFRSF1A	CAGGAAGAACCAGTACCG	TTCTTACAGTTACTACAGGAGAC
TSG-6	CATCTCGCAACTTACAAGC	AGACGGATTCCATAATCAATAATG