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Stem Cells International
Volume 2015 (2015), Article ID 638153, 12 pages
Research Article

Pretreatment of Adipose Derived Stem Cells with Curcumin Facilitates Myocardial Recovery via Antiapoptosis and Angiogenesis

1Department of Geriatric Cardiology, Medical School of Chinese PLA, Chinese PLA General Hospital, Beijing 100853, China
2The First Surgical Department of Nanlou, Chinese PLA General Hospital, Beijing 100853, China
3Department of Cardiology, Shanghai Changning Center Hospital, Shanghai 200336, China
4Institute of Geriatrics, Chinese PLA General Hospital, Beijing 100853, China
5Department of Cardiology, Tenth People’s Hospital, Tongji University, Shanghai 200072, China
6College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing 100044, China

Received 4 November 2014; Revised 28 December 2014; Accepted 29 December 2014

Academic Editor: Mohsin Khan

Copyright © 2015 Jianfeng Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The poor survival rate of transplanted stem cells in ischemic myocardium has limited their therapeutic efficacy. Curcumin has potent antioxidant property. This study investigates whether prior curcumin treatment protects stem cells from oxidative stress injury and improves myocardial recovery following cells transplantation. Autologous Sprague-Dawley rat adipose derived mesenchymal stem cells (ADSCs) were pretreated with or without curcumin. The hydrogen peroxide/serum deprivation (H2O2/SD) medium was used to mimic the ischemic condition in vitro. Cytoprotective effects of curcumin on ADSCs were evaluated. Curcumin pretreatment significantly increased cell viability and VEGF secretion, and decreased cell injury and apoptosis via regulation of PTEN/Akt/p53 and HO-1 signal proteins expression. The therapeutic potential of ADSCs implantation was investigated in myocardial ischemia-reperfusion injury (IRI) model. Transplantation of curcumin pretreated ADSCs not only resulted in better heart function, higher cells retention, and smaller infarct size, but also decreased myocardial apoptosis, promoted neovascularization, and increased VEGF level in ischemic myocardium. Together, priming of ADSCs with curcumin improved tolerance to oxidative stress injury and resulted in enhancement of their therapeutic potential of ADSCs for myocardial repair. Curcumin pretreatment is a promising adjuvant strategy for stem cells transplantation in myocardial restoration.