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Stem Cells International
Volume 2016 (2016), Article ID 1247950, 10 pages
http://dx.doi.org/10.1155/2016/1247950
Research Article

Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma Administration

1Cell Therapy Area, Clínica Universidad de Navarra, Av. de Pío XII 36, 31008 Pamplona, Spain
2Arthroscopic Surgery Unit Research, Hospital Vithas San Jose, C/Beato Tomás de Zumarraga 10, 01008 Vitoria-Gasteiz, Spain
3Center for Applied Medical Research, Av. de Pío XII 55, 31008 Pamplona, Spain
4Fundacion Eduardo Anitua, C/Jose María Cagigal 19, 01007 Vitoria-Gasteiz, Spain
5Arthroscopic Surgery Unit, Hospital Vithas San Jose, C/Beato Tomás de Zumarraga 10, 01008 Vitoria-Gasteiz, Spain
6Orthopaedic Surgery and Traumatology Department, Clínica Universidad de Navarra, Av. de Pío XII 36, 31008 Pamplona, Spain
7Hematology Department, Clínica Universidad de Navarra, Av. de Pío XII 36, 31008 Pamplona, Spain

Received 26 April 2016; Revised 12 September 2016; Accepted 20 September 2016

Academic Editor: Armand Keating

Copyright © 2016 Emma Muiños-López et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to evaluate the effect of intra-articular (IA) or a combination of intra-articular and intraosseous (IO) infiltration of Platelet Rich Plasma (PRP) on the cellular content of synovial fluid (SF) of osteoarthritic patients. Thirty-one patients received a single infiltration of PRP either in the IA space () or in the IA space together with two IO infiltrations, one in the medial femoral condyle and one in the tibial plateau (). SF was collected before and after one week of the infiltration. The presence in the SF of mesenchymal stem cells (MSCs), monocytes, and lymphocytes was determined and quantified by flow cytometry. The number and identity of the MSCs were further confirmed by colony-forming and differentiation assays. PRP infiltration into the subchondral bone (SB) and the IA space induced a reduction in the population of MSCs in the SF. This reduction in MSCs was further confirmed by colony-forming (CFU-F) assay. On the contrary, IA infiltration alone did not cause variations in any of the cellular populations by flow cytometry or CFU-F assay. The SF of osteoarthritic patients contains a population of MSCs that can be modulated by PRP infiltration of the SB compartment.