The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation

<div>Stemness characterization of iPSCs generated by the established protocol. (a) Brightfield image of AP-stained clones. The N3 clone showed the highest number of colonies after 7 days. (b) Colony count of the AP-stained cells in Figure <a href="../fig2/">2</a>(a). Immunocytochemical staining of N1 (c), N2 (d), and N3 (e). N3 showed a slightly higher expression of pluripotency markers than N1 and N2. 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(f) Gene expression of pluripotency markers in each clone was confirmed by RT-PCR. Cells were compared to raw PBMCs and fibroblast-derived iPSCs. Scale bars: 200 <i>μ</i>m.</div>

Stem Cells International

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Figure 2

Figure 2: The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation 

Figure 2 | The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation 