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Stem Cells International
Volume 2016, Article ID 2475631, 14 pages
Research Article

High Content Analysis of Human Pluripotent Stem Cell Derived Hepatocytes Reveals Drug Induced Steatosis and Phospholipidosis

1Takara Bio Europe AB (Former Cellectis AB/Cellartis AB), Arvid Wallgrens Backe 20, 413 46 Göteborg, Sweden
2Novo Nordisk A/S, Stem Cell Development, 2880 Bagsværd, Denmark
3Horizon Discovery Ltd, 7100 Cambridge Research Park, Waterbeach, Cambridge CB25 9TL, UK
4Systems Biology Research Center, School of Bioscience, University of Skövde, 541 28 Skövde, Sweden
5Department of Physiology and Pharmacology, Section of Pharmacogenetics, Karolinska Institutet, 171 77 Stockholm, Sweden
6AstraZeneca R&D, GMD CVMD GMed, 431 83 Mölndal, Sweden
7NovaHep AB, Arvid Wallgrens Backe 20, 413 46 Göteborg, Sweden

Received 30 July 2015; Revised 7 October 2015; Accepted 13 October 2015

Academic Editor: Toon van Veen

Copyright © 2016 Arvind Pradip et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The table is a scoring sheet of the compound induced changes for each HCA parameter at isomolar concentration of 100 µM (except for Amiodarone HCL at 125 µM). The scoring sheet serves to estimate the degree of injury for each compound and is adapted from Tolosa et al. (18). The order of degree of injury which the compounds are classified is compared between hiPSC derived hepatocytes (hiPS HEP) and HepG2 cell lines.

  1. Supplementary Material