Semaphorin 3A Shifts Adipose Mesenchymal Stem Cells towards Osteogenic Phenotype and Promotes Bone Regeneration In Vivo

Figure 7

Sema3A significantly activates Wnt/β-catenin pathway in ASCs. (a) Conditioned medium from the Sema3A infected cells increased the migrated cells number. (b) Quantitative analysis of migrated cells in Figure 7(a). (c) No significant differences were found in the proliferation rate between and . (d) Immunofluorescence staining of β-catenin in control and Sema3A overexpressed ASCs, and Sema3A induced nuclear translocation of β-catenin. (e) Sema3A overexpression significantly increased the Topflash/Fopflash ratio, indicating activation of Wnt pathway. (f, g, and h) The mRNA expression analysis of the Wnt pathway related genes and downstream transcriptional targets of β-catenin in control and Sema3A overexpressed ASCs. Mean ± SD, , and . CM, conditioned medium.

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