Research Article
Endothelium-Independent Hypoxic Contraction Is Prevented Specifically by Nitroglycerin via Inhibition of Akt Kinase in Porcine Coronary Artery
Figure 1
Effects of NTG, ISO, DETA NONOate, and cGMP on hypoxic vasoconstriction. The continuous contraction caused by hypoxia in porcine coronary arteries was prevented by NTG (10−6 M), but not by isoproterenol (ISO, 10−7 M; (a)). The prevention of the hypoxic vasoconstriction caused by NTG (10−6 M; (b)), DETA NONOate (NO, 10−4 M; (c)), and 8-Br-cGMP (cGMP, 10−4 M; (d)) was recovered by ODQ (3 × 10−5 M, a specific inhibitor of sGC; (b) and (c)) and Rp-Br-PET-cGMPS (PKG-I; 3 × 10−5 M; (d)). The restored hypoxic contraction of porcine coronary arteries by ODQ or Rp-Br-PET-cGMPS was reversed by LY294002 (10−5 M, a specific inhibitor of PI3K; (b), (c), and (d)). Data are means ± SEM; –6 for each group. different between the control group and treatment group with NTG, DETA NONOate, or 8-Br-cGMP (); different between the group with LY294002 and the group without LY294002 ().
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