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Stem Cells International
Volume 2016, Article ID 3826249, 14 pages
Research Article

High-Fidelity Reprogrammed Human IPSCs Have a High Efficacy of DNA Repair and Resemble hESCs in Their MYC Transcriptional Signature

1Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
2Institute for Cell Engineering and Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

Received 18 March 2016; Revised 23 June 2016; Accepted 14 July 2016

Academic Editor: Silvia Brunelli

Copyright © 2016 Pratik K. Nagaria et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Schematic of the I-Sce1 EJ5 plasmid reactivation assay. Analysis of the repair products from the EJ5-I-Sce1-based end-joining assay.pimEJ5GFP was linearized using I-Sce1 and incubated with the dialyzed nuclear extracts from the respective cell lines. GFP genes were PCR amplified from the in vitro ligation reaction.

Supplementary Figure 2: Phosphorylated p53 normalization to total p53 protein. Densitometry analysis of the Western blots for measurement of phosphorylated p53Ser15 (p-p53) plotted relative to total p53 (alternate analysis of Fig 3C and Fig 3D), using ImageJ software. Statistical significance of the data was determined using 2-way ANOVA analysis with Bonferroni post-tests to compare the replicates (three-independent experiments).

  1. Supplementary Material