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Stem Cells International
Volume 2016, Article ID 5481493, 12 pages
http://dx.doi.org/10.1155/2016/5481493
Research Article

Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes

1Department of Cellular Biotechnologies and Hematology, Section of Molecular Genetics, Sapienza University of Rome, Viale Regina Elena, 324, 00161 Rome, Italy
2National Institute for Infectious Diseases L. Spallanzani, IRCCS, Via Portuense 292, 00149 Rome, Italy

Received 28 July 2015; Revised 12 October 2015; Accepted 13 October 2015

Academic Editor: Malin Jonsson

Copyright © 2016 Angela Maria Cozzolino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. (A) and (B) RT–qPCR analysis for the indicated genes of RLSCs grown on 0,4 kPa and 80 kPa at 24 hours (upper panels) and 48 hours (lower panels) in two independent experiments. Data are expressed as fold change in gene expression in cells grown on hydrogels versus CTRL (arbitrary value = 1). Note the logarithmic scale.

Supplementary Figure 2. RT–qPCR analysis of Cyp2b10 in RLSCs (A), MMH/E14 (B) and WT/3A (C) grown on plastic (CTRL) and on 0,4 kPa hydrogel at the indicated times. Data are expressed as fold change in gene expression in cells grown on hydrogels versus CTRL (arbitrary value = 1).

  1. Supplementary Material