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Stem Cells International
Volume 2016, Article ID 5934782, 9 pages
Review Article

iPSCs: A Minireview from Bench to Bed, including Organoids and the CRISPR System

Basic Science and Experimental Medicine Institute, University Institute of the Italian Hospital, Juan D. Perón 4190, C1181ACH Buenos Aires, Argentina

Received 23 August 2015; Revised 19 October 2015; Accepted 25 October 2015

Academic Editor: Xuekun Li

Copyright © 2016 Andrés Javier Orqueda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


When Dolly the sheep was born, the first probe into an adult mammalian genome traveling back in time and generating a whole new animal appeared. Ten years later, the reprogramming process became a defined method of producing induced pluripotent stem cells (iPSCs) through the overexpression of four transcription factors. iPSCs are capable of originating virtually all types of cells and tissues, including a whole new animal. The reprogramming strategies based on patient-derived cells should make the development of clinical applications of cell based therapy much more straightforward. Here, we analyze the current state, opportunities, and challenges of iPSCs from bench to bed, including organoids and the CRISPR system.