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Stem Cells International
Volume 2016, Article ID 6429853, 11 pages
Research Article

p53-Dependent Senescence in Mesenchymal Stem Cells under Chronic Normoxia Is Potentiated by Low-Dose γ-Irradiation

1Institute of Radiation Biology, Helmholtz Center Munich, German Research Center for Environmental Health, Ingolstaedter Landstraße 1, 85764 Neuherberg, Germany
2Chair of Radiation Biology, Technical University Munich, Ismaninger Straße 22, 81675 Munich, Germany
3Research Group Clinical Biochemistry, Medical Clinic and Polyclinic IV, Medical Center of the University of Munich, Schillerstraße 42, 80336 Munich, Germany

Received 31 July 2015; Accepted 2 November 2015

Academic Editor: Jane Synnergren

Copyright © 2016 Ines Höfig et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary material contains an overview over the tri-lineage differentiation potential of in vitro cultured p53 wild-type mMSCs to form osteocytes, chondrocytes and adipocytes (S1). Multiplex quantification of viability (S2a), cytotoxicity (S2b) and apoptosis induction (S2c) of mMSCs after γ-irradiation under normoxia and hypoxia is shown in the next figure including apoptosis- and necrosis-inducing agents as positive controls. Supplementary Figure S3 describes the effects of oxygen levels on p53 protein stabilization measured by immunohistochemistry in sham-irradiated and 4 Gy irradiated mMSCs, p53-deficient mMSCs served as negative controls. Colony formation in p53wt/wt (a) and p53-/- mMSCs (b) under hypoxic and normoxic conditions as basis for CFU-F calculation is shown in supplementary figure S4.

  1. Supplementary Material