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Stem Cells International
Volume 2016, Article ID 7403890, 12 pages
http://dx.doi.org/10.1155/2016/7403890
Research Article

Implant Composed of Demineralized Bone and Mesenchymal Stem Cells Genetically Modified with AdBMP2/AdBMP7 for the Regeneration of Bone Fractures in Ovis aries

1Departmento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico
2Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, Mexico
3Banco de Huesos y Tejidos, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, Mexico
4Departmento de Morfología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico
5Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León, Monterrey, Mexico
6Servicio de Ortopedia y Traumatología, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, Mexico
7Servicio de Radiología e Imagen, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, Mexico

Received 30 April 2016; Accepted 29 June 2016

Academic Editor: Bin Li

Copyright © 2016 Adelina A. Hernandez-Hurtado et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Adipose-derived mesenchymal stem cells (ADMSCs) are inducible to an osteogenic phenotype by the bone morphogenetic proteins (BMPs). This facilitates the generation of implants for bone tissue regeneration. This study evaluated the in vitro osteogenic differentiation of ADMSCs transduced individually and in combination with adenoviral vectors expressing BMP2 and BMP7. Moreover, the effectiveness of the implant containing ADMSCs transduced with the adenoviral vectors AdBMP2/AdBMP7 and embedded in demineralized bone matrix (DBM) was tested in a model of tibial fracture in sheep. This graft was compared to ewes implanted with untransduced ADMSCs embedded in the same matrix and with injured but untreated animals. In vivo results showed accelerated osteogenesis in the group treated with the AdBMP2/AdBMP7 transduced ADMSC graft, which also showed improved restoration of the normal bone morphology.