Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool for In Vitro Osteogenesis and Bone Tissue Engineering Approaches

Figure 2

Correlation of TNAP inducibility to endogenous TNAP activity and frequency of certain inducibility values. hBMSC in passage 1 were plated in BM (basic medium) onto TCPS (= P2). From day 4 after plating, the cells were cultured either in BM or in OM/D (osteogenic differentiation medium) and analyzed at day 15 after plating for TNAP activity as described. The inducibility of TNAP activity (TNAP in OM/D : TNAP in BM) was calculated from individual donor data pairs and plotted against endogenous TNAP activity (a). The black line indicates a cubic curve fit; -square values are given in Table 2; (all donors), (females), and (males). By plotting the number of donors with a given TNAP inducibility, the frequency of certain inducibility value was figured out (b). The data points were analyzed by nonlinear curve fit and resulted in a Gaussian distribution. The peak characteristics () are indicated in the graphs; (all donors), (females), and (males).

(a)

(b)