Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool for In Vitro Osteogenesis and Bone Tissue Engineering Approaches

Figure 3

Correlation of donor age with TNAP activity and inducibility. hBMSC in passage 1 were plated in BM (basic medium) onto TCPS (= P2). From day 4 after plating, the cells were cultured either in BM or in OM/D (osteogenic differentiation medium). At day 15 after plating, the cells were analyzed for TNAP activity as described above. Data for endogenous TNAP activity, TNAP activity in OM/D, and TNAP inducibility for each donor age group were plotted each in one graph. The lines indicate linear curve fit. Regression characteristics (-value, -square) and the number of analyzed donors are given in Table 2.