Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways

Figure 3

Polydatin attenuated H₂O₂-induced apoptosis in BMSCs. (a) Hochest 33258 staining was applied to detect the nuclear condensation of BMSCs, pretreated with polydatin in presence of H₂O₂. Fluorescence images (A–D) were observed by fluorescence microscope. (A)–(D) represented CT, polydatin, H₂O₂, and H₂O₂ + polydatin group, respectively. (b) BMSCs were pretreated with 30 μM polydatin for 2 h and followed by exposing to H₂O₂ (600 μM) for 12 h. The induction of apoptosis was determined using Annexin V-FITC/PI staining. (c) Quantitative analysis of apoptotic cells in Figure 3(b). (d) The expression of Bcl-2, cleaved caspase-3, and Bax of BMSCs exposed to H₂O₂ with or without polydatin. Data are presented as means ± S.D. versus control group; versus H₂O₂-treated group.

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