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Stem Cells International
Volume 2017, Article ID 2353240, 16 pages
Research Article

Crosstalk with Inflammatory Macrophages Shapes the Regulatory Properties of Multipotent Adult Progenitor Cells

1Biomedical Research Institute/Transnational University Limburg, School of Life Sciences, Hasselt University, 3590 Diepenbeek, Belgium
2Department of Regenerative Medicine, Athersys Inc., Cleveland, OH, USA
3ReGenesys BVBA, Leuven, Belgium

Correspondence should be addressed to Niels Hellings; eb.tlessahu@sgnilleh.slein

Received 9 March 2017; Revised 27 May 2017; Accepted 12 June 2017; Published 12 July 2017

Academic Editor: Vladislav Volarevic

Copyright © 2017 Stylianos Ravanidis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental figures Supplemental Figure 1: Schematic illustration of generation of conditioned media from rMAPC and application to macrophages (MΦ). Supplemental Figure 2: Macrophage-primed rMAPC increase mRNA expression of chemokines. CCL2, CCL5 and CXCL10 mRNA expression in rMAPC treated with SN of LPS-activated macrophages, SN of naïve macrophages (-LPS-SN) or LPS. Results are shown as fold differences in comparison to SN of naïve macrophages. Expression of target genes was normalized against expression of 14-3-3 protein zeta/delta (YWHAZ) and TATA-binding protein (TBP). Mean values ± SEM are from 5 experiments. Asterisks (∗) indicate statistical significant difference with SN of naïve macrophages. Data were analyzed with one way ANOVA followed by Dunnett’s multiple comparison test. ∗p≤0.05, ∗∗p≤0.01 and ∗∗∗p≤0.001.

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