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Stem Cells International
Volume 2017, Article ID 5706193, 13 pages
Research Article

Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in Rats

1Department of Physiology, Hebei Medical University, Shijiazhuang, Hebei 050017, China
2Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, Hebei 050017, China
3Department of Cardiology, Bethune International Peace Hospital, Shijiazhuang, Hebei 050017, China
4Undergraduate of College of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, China
5Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, China
6Cell Therapy Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China
7Department of Immunology, Basic Medical College, Hebei Medical University, Shijiazhuang, Hebei 050017, China

Correspondence should be addressed to Yi Zhang; nc.anis@yrnehyhz, Chuan Wang; moc.liamtoh@ahphcw, and Quanhai Li; moc.liamtoh@5020iliahnauq

Received 27 January 2017; Revised 27 April 2017; Accepted 8 May 2017; Published 16 July 2017

Academic Editor: Eftekhar Eftekharpour

Copyright © 2017 Jing Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aplastic anemia (AA) is a common malignant blood disease, and chronic intermittent hypobaric hypoxia (CIHH) has a beneficial effect against different diseases. The aim of the present study was to investigate the protective effect of CIHH against AA and underlying mechanisms. 5-Fluorouracil and busulfan treatment induced AA model in rats with reduction of hematological parameters and bone marrow tissue injury and decrease of the colony numbers of progenitor cells. CIHH pretreatment significantly reduced the incidence rate of AA and alleviated above symptoms in AA model. The adhesive molecules of bone marrow mesenchymal stem cells (BMMSCs) in AA model, VLA-4, VCAM-1, and ICAM-1 were upregulated, and those of CD162 and CD164 were downregulated by CIHH pretreatment. The expressions of HIF-1α and NF-κB in BMMSCs were also decreased through CIHH pretreatment. Overall, the results demonstrated for the first time that CIHH has an anti-AA effect through improving the adhesiveness and stress of mesenchymal stem cells in rats. CIHH could be a promising and effective therapy for AA.