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Stem Cells International
Volume 2017 (2017), Article ID 5804147, 10 pages
Research Article

IGF-1 Gene Transfer to Human Synovial MSCs Promotes Their Chondrogenic Differentiation Potential without Induction of the Hypertrophic Phenotype

1Department of Orthopaedic Surgery, School of Medicine, Sapporo Medical University, Sapporo, Japan
2Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, Suita, Japan
3McCaig Institute for Bone and Joint Health, University of Calgary, Calgary, AB, Canada
4Department of Orthopedic Surgery, Saarland University Medical Center, Homburg, Saar, Germany
5Global Center for Medical Engineering and Informatics, Osaka University, Suita, Japan
6Institute for Medical Science in Sports, Osaka Health Science University, Osaka, Japan

Correspondence should be addressed to Norimasa Nakamura

Received 28 February 2017; Accepted 7 May 2017; Published 27 June 2017

Academic Editor: Leonard M. Eisenberg

Copyright © 2017 Yasutoshi Ikeda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Information. Supplemental Figure 1: The leniviral-mediated gene intduction of IGF-1 into the human Syn-MSC. A: showing mRNA transcript levels by RT-PCR using human IGF-1 specific primer pairs. The control (left) vs. IGF-1 gene transferred cell (right). B: Protein expression by Western blotting with anti-IGF1 antibody (ab9572, Abcam, Cambridge, UK). The control (left) vs. IGF-1 gene transferred cell (right). C: Cell surface marker expression of Control and IGF-1 transferred cells by fluorescence-activated cell sorting (FACS) analysis. Supplemental Figure 2: The results of histology assessments from the other cases. The control (left) vs. IGF-1 gene transferred cell derived pellets (right). T-B; Toluidine-Blue (upper lane),S-O; Safranin-O (lower lane). Left: Scale bars =500μm, right: Scale bars =200 μm.

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