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Stem Cells International
Volume 2017 (2017), Article ID 6082684, 11 pages
Research Article

hTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System

1Department of Anesthesiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
2Department of Anesthesiology, The Third Xiangya Hospital of Central South University, Changsha 410013, China
3Laboratory of Research Center for Translational Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
4Department of Anesthesiology, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China

Correspondence should be addressed to Ke An

Received 21 November 2016; Revised 26 January 2017; Accepted 22 February 2017; Published 11 May 2017

Academic Editor: Veronica Ramos-Mejia

Copyright © 2017 Ke An et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Legends of Supplemental Figures. Figure S1. RT-PCR for hTERT mRNA expression from cells in vitro. The up-regulated expression of hTERT mRNA in BMSCs transfected with pLV.ExSi/Puro-EF1α-hTERT compared with BMSCs transfected with empty vector pLV.ExSi/PGK-Puro or BMSCs without transfection. BMSCs, PGK-BMSCs, hTERT-BMSCs and DL–1000 DNA ladder (lanes 1–4). GAPDH was used as an internal reference. Figure S2. Growth and proliferation feature assay of hTERT-BMSCs. (A) Growth curvesof BMSCs, PGK-BMSCs and hTERT-BMSCs. (B) Cell cycle distribution of BMSCs and BMSCs transfected with control or hTERT. Significance level is P < 0.05, indicated by ∗. Figure S3. Karyotype analysis of hTERT-BMSCs. hTERT-BMSCs displayed the same chromosomal pattern as their parental cells (diploid number 42) after genetic modification to express exogenous hTERT. Figure S4. Identification of rat GAL cDNA (A) and recombinant transformants (B) by PCR.

  1. Supplementary Material