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Stem Cells International
Volume 2017, Article ID 7859184, 7 pages
Research Article

Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells

1Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of Korea
2Department of Chemical Engineering, Hanyang University, Seoul, Republic of Korea
3AmorePacific Corp./R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea
4Predictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of Korea
5Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon, Republic of Korea
6Department of Dentistry, Korea University, Ansan Hospital, Ansan, Republic of Korea

Correspondence should be addressed to Sung-Hwan Moon; and Sun-Woong Kang;

Received 5 August 2016; Revised 28 November 2016; Accepted 6 December 2016; Published 19 January 2017

Academic Editor: Eftekhar Eftekharpour

Copyright © 2017 Taekhee Jung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Differentiation of stem cells is an important strategy for regeneration of defective tissue in stem cell therapy. Bone morphogenetic protein-2 (BMP-2) is a well-known osteogenic differentiation factor that stimulates stem cell signaling pathways by activating transmembrane type I and type II receptors. However, BMPs have a very short half-life and may rapidly lose their bioactivity. Thus, a BMP delivery system is required to take advantage of an osteoinductive effect for osteogenic differentiation. Previously, BMP delivery has been designed and evaluated for osteogenic differentiation, focusing on carriers and sustained release system for delivery of BMPs. The effect of the delivery mode in cell culture plate on osteogenic differentiation potential was not evaluated. Herein, to investigate the effect of delivery mode on osteogenic differentiation of BM-MSCs in this study, we fabricated bottom-up release and top-down release systems for culture plate delivery of BMP-2. And also, we selected Arg-Gly-Asp- (RGD-) conjugated alginate hydrogel for BMP-2 delivery because alginate is able to release BMP-2 in a sustained manner and it is a biocompatible material. After 7 days of culture, the bottom-up release system in culture plate significantly stimulated alkaline phosphate activity of human bone marrow-mesenchymal stem cells. The present study highlights the potential value of the tool in stem cell therapy.