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Stem Cells International
Volume 2017 (2017), Article ID 7859184, 7 pages
Research Article

Effect of BMP-2 Delivery Mode on Osteogenic Differentiation of Stem Cells

1Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Republic of Korea
2Department of Chemical Engineering, Hanyang University, Seoul, Republic of Korea
3AmorePacific Corp./R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea
4Predictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of Korea
5Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon, Republic of Korea
6Department of Dentistry, Korea University, Ansan Hospital, Ansan, Republic of Korea

Correspondence should be addressed to Sung-Hwan Moon and Sun-Woong Kang

Received 5 August 2016; Revised 28 November 2016; Accepted 6 December 2016; Published 19 January 2017

Academic Editor: Eftekhar Eftekharpour

Copyright © 2017 Taekhee Jung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In Supplementary Figure 1, BM-MSCs were cultivated up to five passages and we subsequently examined representative MSC markers by FACS analysis. MSC specific markers, CD 44 (95.39%), CD 73 (93.13%), CD 90 (98.60%), and CD 105 (94.71%), were strongly expressed in the cultured population. This result showed that the differentiated cells expressed relevant MSC markers which indicate that the cells possessed MSC characteristics. In Supplementary Figure 2, since MSCs induced towards an osteogenic fate are known to strongly express ALP, BCA protein assay reagent was used to normalize ALP activity by the protein content for examination. BM-MSCs cultured on RGD-modified alginate without BMP-2 were used as negative control.

  1. Supplementary Material