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Stem Cells International
Volume 2017 (2017), Article ID 9303598, 11 pages
https://doi.org/10.1155/2017/9303598
Research Article

Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors

1INSERM, UMR 1229, Regenerative Medicine and Skeleton (RMeS), Université de Nantes, ONIRIS, 44042 Nantes, France
2UFR Sciences Biologiques et Pharmaceutiques, Université de Nantes, 44035 Nantes, France
3UFR Odontologie, Université de Nantes, 44042 Nantes, France
4CHU Nantes, Pharmacie Centrale, PHU 11, 44093 Nantes, France
5INSERM, UMS 016, CNRS 3556, Structure Fédérative de Recherche François Bonamy, Micropicell Facility, CHU Nantes, Université de Nantes, 44042 Nantes, France
6CHU Nantes, PHU 4 OTONN, 44093 Nantes, France

Correspondence should be addressed to Jérôme Guicheux

Received 25 May 2017; Revised 3 August 2017; Accepted 8 August 2017; Published 11 October 2017

Academic Editor: Celeste Scotti

Copyright © 2017 Fahd Hached et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

While therapeutically interesting, the injection of MSCs suffers major limitations including cell death upon injection and a massive leakage outside the injection site. We proposed to entrap MSCs within spherical particles derived from alginate, as a control, or from silanized hydroxypropyl methylcellulose (Si-HPMC). We developed water in an oil dispersion method to produce small Si-HPMC particles with an average size of about 68 μm. We evidenced a faster diffusion of fluorescein isothiocyanate-dextran in Si-HPMC particles than in alginate ones. Human adipose-derived MSCs (hASC) were encapsulated either in alginate or in Si-HPMC, and the cellularized particles were cultured for up to 1 month. Both alginate and Si-HPMC particles supported cell survival, and the average number of encapsulated hASC per alginate and Si-HPMC particle (7102 and 5100, resp.) did not significantly change. The stimulation of encapsulated hASC with proinflammatory cytokines resulted in the production of IDO, PGE2, and HGF whose concentration was always higher when cells were encapsulated in Si-HPMC particles than in alginate ones. We have demonstrated that Si-HPMC and alginate particles support hASC viability and the maintenance of their ability to secrete therapeutic factors.