Icariin Promotes the Migration of BMSCs In Vitro and In Vivo via the MAPK Signaling Pathway

Figure 3

ICA promotes the migration of BMSCs in vitro. (a) Scratch wound-healing assay of BMSCs treated with ICA (0, 0.1, 1, 10, and 100 μM). Phase contrast images were captured after 12 and 24 h. (b) Quantitative analysis of the remaining wound area in Figure 3(a). Three random fields of each group were selected, and the remaining area of wound was measured using Image J. (c) Transwell migration assay of BMSCs treated with ICA (0.1, 1, and 10 μM). The number of BMSCs in the outside bottom chamber was calculated. (d) Quantitative analysis of the migrated cells. The results are shown as the mean value of 5 random fields. (e) ICA stimulated actin stress fiber formation of BMSCs. Data are shown as the mean ± SD of three independent experiments. , compared with the group control.

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