Oxygen Regulates Human Pluripotent Stem Cell Metabolic Flux

Figure 5

RNA-seq zero-order networks in hPSC due to 5% and 20% oxygen culture. (a) MEL1 hPSC differentially expressed genes due to oxygen were submitted to NetworkAnalyst and connected based on known protein:protein interactions. Red nodes are upregulated at 5% oxygen; green nodes are upregulated at 20% oxygen. Green/red intensity indicates the degree of fold change as indicated in the figure. (b) MEL1 hPSC KEGG pathways upregulated at 5% and 20% oxygen based on the zero-order network in Figure 5(a). (c) MEL2 hPSC zero-order and protein:protein interaction network. (d) MEL2 hPSC KEGG pathways upregulated at 5% and 20% oxygen based on the zero-order network in (c).

(a)

(b)

(c)

(d)