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Stem Cells International
Volume 2019, Article ID 9640790, 20 pages
Research Article

Comparative Analysis of Biological Properties of Large-Scale Expanded Adult Neural Crest-Derived Stem Cells Isolated from Human Hair Follicle and Skin Dermis

1Department of Genetic Diagnostics, State Institute of Genetic and Regenerative Medicine NAMS of Ukraine, 67 Vyshgorodska Str., Kyiv 04114, Ukraine
2Biotechnology Laboratory ilaya.regeneration, Medical Company ilaya®, 9 I. Kramskogo Str., Kyiv 03115, Ukraine
3Department of Functional Genomics, Institute of Molecular Biology and Genetics NAS of Ukraine, 150 Zabolotnogo Str., Kyiv 03143, Ukraine
4Department of Molecular and Cellular Pathobiology, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, 45 Vasylkivska Str., Kyiv 03022, Ukraine
5Basic Laboratory of Immunology and Cell Biotechnologies, Immanuel Kant Baltic Federal University, Kaliningrad 236041, Russia
6Department of Cell and Tissue Technologies, State Institute of Genetic and Regenerative Medicine NAMS of Ukraine, 67 Vyshgorodska Str., Kyiv 04114, Ukraine

Correspondence should be addressed to Roman G. Vasyliev; moc.liamg@ayali.veilysavr

Received 23 August 2018; Revised 14 October 2018; Accepted 22 November 2018; Published 19 February 2019

Academic Editor: Hans-Günther Machens

Copyright © 2019 Roman G. Vasyliev et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Introduction. The adult neural crest-derived stem cells (NCSCs) have significant perspectives for use in regenerative medicine. The most attractive sources for adult NCSC isolation are the hair follicles (HF) and skin dermis (SD) because of easy access and minimally invasive biopsy. The aim of this study was to compare the biological properties of HF- and SD-derived NCSCs after their large-scale expansion. Methods. The conventional explant method was used to obtain HF NCSCs. For the isolation of SD NCSCs, a new combined technique consisting of preplating and subsequent culturing in 3D blood plasma-derived fibrin hydrogel was applied. The studied cells were characterized by flow cytometry, ICC, qPCR, Bio-Plex multiplex assay, and directed multilineage differentiation assays. Results. We have obtained both adult SD and HF NCSCs from each skin sample (). Adult SD and HF NCSCs were positive for key neural crest markers: SOX10, P75 (CD271), NESTIN, SOX2, and CD349. SD NCSCs showed a higher growth rate during the large-scale expansion compared to HF NCSCs (). Final population of SD NCSCs also contained more clonogenic cells () and SOX10+, CD271+, CD105+, CD140a+, CD146+, CD349+ cells (). Both HF and SD NCSCs had similar gene expression profiling and produced growth factors, but some quantitative differences were detected. Adult HF and SD NCSCs were able to undergo directed differentiation into neurons, Schwann cells, adipocytes, and osteoblasts. Conclusion. The HF and SD are suitable sources for large-scale manufacturing of adult NCSCs with similar biological properties. We demonstrated that the NCSC population from SD was homogenous and displayed significantly higher growth rate than HF NCSCs. Moreover, SD NCSC isolation is cheaper, easier, and minimally time-consuming method.