Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors

Figure 5

PAX expression level and distribution pattern of human iPSC-derived myogenic progenitors enriched using identified markers. (a) Representative ICC images of PAX7 expression (stained green) in undifferentiated cells of sorted human iPSC-derived EZ spheres. Long yellow arrows indicate nuclear localization of PAX7. Pink arrowheads indicate cytoplasmic distribution of PAX7. . (b) Means of % PAX7⁺ cells in undifferentiated cultures of sorted fractions. Error bars represent SEM from two independent experiments. No statistical significance as determined by one-way ANOVA. (c) Means of % cells with PAX7 expression localized in the nuclei and % cells with PAX7 expression scattered across the cytoplasm among PAX7⁺ cells in undifferentiated cultures of sorted fractions. Error bars represent SEM from two independent experiments. Statistical significance was calculated by one-way ANOVA followed by Bonferroni’s post hoc test for pairwise comparisons. , . (d) Means of % nuclear PAX7⁺ cells in undifferentiated cultures of sorted fractions. Error bars represent SEM from two independent experiments. Statistical significance was calculated by one-way ANOVA followed by Bonferroni’s post hoc test for pairwise comparisons. , . (e) Adapted from data in Figure 3(d), means of % MyHC⁺ cells in differentiated cultures of sorted fractions. Error bars represent SEM from three independent experiments. Statistical significance for pairwise comparisons was calculated by unpaired two-tailed Student’s -test. , , and .

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