Exosomes Derived from Runx2-Overexpressing BMSCs Enhance Cartilage Tissue Regeneration and Prevent Osteoarthritis of the Knee in a Rabbit Model

Figure 6

YAP plays an important role in mediating the effects of BMSCs-Exos stimulation. (a, b) Gene expression changes of aggrecan, COL-II, SOX9, YAP, ANKRD1, and CTGF after silence by YAP. The mark on the horizontal line applies to Figure 6. (c, d) Protein expression levels of aggrecan, COL-II, SOX9, YAP, ANKRD1, and CTGF were detected by western blotting after silence by YAP, and the results of statistical analysis also shown. (e, f) The number of invaded rbACs measured by transwell assay, and the results of statistical analysis also shown. Original magnification, ×200. (g) Proliferation of rbACs determined by EdU labeling assay. Original magnification, ×200. (h) Quantitative analysis for EdU-positive cells. This experiment was repeated three times. ; compared to NC; ^&; ^&& compared to Exos.

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