Characterization of Nora Virus Structural Proteins via Western Blot Analysis

Figure 3

Western blot analysis of Nora virus proteins and recombinant proteins. Mouse antiserum was prepared against whole Nora virus particles (lower band virus particles, see Figure 1) as described in Section 2. Nora virus purified on CsCl gradients was separated into upper band particles (virus UB) and lower band particles (virus LB). The viral protein components of the UB and LB particles were separated on 10% SDS-PAGE gels and blotted onto nitrocellulose membranes, along with the E. coli expressed recombinant Nora virus proteins (VP3, VP4a, VP4b, VP4c, and ORF1). The recombinant proteins were either strongly detected (VP3, VP4a, and VP4c), weakly detected (VP4b), or not detected at all (ORF1). There does not appear to be any discernable polypeptide composition differences between the upper and lower band virus particles.