Characterization of Nora Virus Structural Proteins via Western Blot Analysis

Figure 4

Western blot analysis of the polypeptide composition of the upper band and lower band Nora virus particles using monospecific antisera. Mice were hyperimmunized with rVP3, rVP4a, rVP4b, or rVP4c and the resultant sera were collected as described in Section 2. (a) shows the results of using anti-VP3 antiserum. An SDS-PAGE gel was loaded with molecular weight markers (MW), recombinant VP3 (rVP3), 5.55 μg of upper band virus particles (UB), 5.55 μg of lower band virus particles (LB), Nora virus infected fly lysate (Dm-I), or Nora virus uninfected fly lysate (Dm-U). After electrophoretic separation, the polypeptides were blotted onto a nitrocellulose membrane and developed as described in Figure 4 using a 1 : 1,000 dilution of the primary antibody. (b) (VP4a), (c) (VP4b), and (d) (VP4c) were all run identically to (a) with the exception of the dilution of the primary antibody which was 1 : 500, 1 : 2,000, and 1 : 500, for VP4a, VP4b, and VP4c, respectively.

(a) VP3

(b) VP4a

(c) VP4b

(d) VP4c