Stroke Status Evoked Adhesion Molecule Genetic Alterations in Astrocytes Isolated from Stroke-Prone Spontaneously Hypertensive Rats and the Apigenin Inhibition of Their Expression
Figure 7
The effects of H/R on the expression levels of adhesion molecules in astrocytes isolated from SHRSP/IZM and a comparison of the inhibitory effects of apigenin and resveratrol, ebselen, NAC, and pioglitazone. The astrocytes isolated from SHRSP/IZM were incubated under hypoxia (1% O2, 24 hour) or hypoxia + reoxygenation (21% O2, 3 hour) as previously described [13, 15]. They were incubated for an additional period either before or after the hypoxia with 50 M of apigenin, resveratrol, ebselen, NAC, or pioglitazone. The ICAM-1, MCP-1, and VCAM-1 mRNA measurements were carried out as described in Figure 1 and in the text. The bars represent the mean ± SD (n = 4). Asterisk (*): compared with the normal condition values. Nor: normal conditions; Hyp: Hypoxia; H/R: hypoxia and reoxygenation.
