A Universal and Efficient Detection of Chytridiomycosis Infections in Amphibians Using Novel Quantitative PCR Markers

Figure 2

(a) Standard curve constructed for dilutions of ITS copy numbers from 10⁸ copies per reaction to 1 copy/reaction (b) An LoD-LoQ Assay performed using 23 replicates each of lower concentration of 1024, 256, 64, 16, 4 and 1 ITS copies per reaction, along with standards starting from 10⁸copies per reaction to 1 copy per reaction (Figure 2(a)) to calculate the lowest standard that can be detected with 95% confidence (LoD) and the lowest quantity that can be amplified with less than 35% covariance.

(a)

(b)