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The Scientific World Journal
Volume 2012, Article ID 539316, 9 pages
http://dx.doi.org/10.1100/2012/539316
Research Article

Expressions of MMPs and TIMP-1 in Gastric Ulcers May Differentiate H. pylori-Infected from NSAID-Related Ulcers

1Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, 138 Sheng Li Road, Tainan 70403, Taiwan
2Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 138 Sheng Li Road, Tainan 70403, Taiwan
3Department of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 138 Sheng Li Road, Tainan 70403, Taiwan
4Department of Pathology, Ton-Yen General Hospital, 69 Xian Zheng 2nd Road, Zhubei, Hsinchu 30268, Taiwan
5Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 138 Sheng Li Road, Tainan 70403, Taiwan

Received 7 December 2011; Accepted 3 January 2012

Academic Editor: Konstantinos Karmiris

Copyright © 2012 Hsiu-Chi Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Two major causes of gastric ulcers are Helicobacter pylori (H. pylori) infection and nonsteroidal anti-inflammatory drug (NSAID) use. Aims. This study aimed to determine if there were different expressions of matrix metalloproteinases (MMPs) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) between H. pylori-infected and NSAID-related ulcers. Methods. The 126 gastric ulcer patients (H. pylori infected 𝑛 = 4 6 ; NSAID related 𝑛 = 3 0 ; combined with two factors 𝑛 = 5 0 ) provided ulcer and nonulcer tissues for assessment of MMP-3, -7, and -9 and TIMP-1 expression by immunohistochemical staining. Results. Gastric ulcer tissues had significantly higher MMP-3, -7, and -9 and TIMP-1 expressions than nonulcer tissues ( 𝑃 < 0 . 0 5 ). H. pylori-infected gastric ulcers had even higher MMP-7, MMP-9, and TIMP-1 expressions in epithelial cells than NSAID-related gastric ulcers ( 𝑃 < 0 . 0 5 ). In patients with the two combined factors, gastric ulcers expressed similar proportions of antral ulcers and MMP-7 and MMP-9 intensities to NSAID-related gastric ulcers, but lower MMP-9 and TIMP-1 than H. pylori-infected gastric ulcers ( 𝑃 < 0 . 0 5 ). Conclusions. H. pylori-infected gastric ulcers express higher MMP-7, MMP-9, and TIMP-1 than NSAID-related ulcers. In patients with the two combined factors, ulcer location and MMP-7 and MMP-9 intensities are similar to NSAID use.