RNAi-Mediated Knockdown of IKK1 in Transgenic Mice Using a Transgenic Construct Containing the Human H1 Promoter
Figure 3
Expression of IKK1 siRNA in transgenic mice. (a) Upper part: representative examples of the northern blots performed for analysis of IKK1 siRNA expression in organs from H1-IKKα-siRNA transgenic lines. Lane C is a positive control that contains RNA from the transfected cells loaded in the first lane of Figure 1(b). The black arrow indicates the position of the specific 21-nucleotide band. Samples from wild-type animals gave no signals for IKK1 siRNA. The motilities of 20-, 30-, 40-, and 50-nucleotide RNA fragments are indicated. Lower part: the same blots were hybridized with a probe specific for U6 RNA, as a loading control. (b) Relative expression levels of IKK1 siRNA in different transgenic lines; siRNA intensities were normalized against their corresponding U6 signals and presented as a percentage of the value obtained for brain in line L1. (c) Relative expression level of IKK1 siRNA in organs of L1 transgenic mice. Signals obtained with an U6 probe were used for normalization. (d) H1 expression in control mice. The same blot was hybridized with a 7S-specific probe, as a loading control. In the histogram of the right, the relative expression of H1 normalized against 7S signals in different organs of control mice is shown. B: brain; Bs: back skin; H: heart; K: kidney; L: liver; Lu: lung; M: muscle; P: pancreas; Pr: prostate; S: spleen; St: stomach; T: thymus; Ts: tail skin.
